What Challenges are Associated with MFA in Histology?
One of the significant challenges is the heterogeneity of tissues. Different cell types within a tissue can have distinct metabolic profiles, which complicates the analysis. Isolating specific cell types or using single-cell techniques can help address this issue. Another challenge is the spatial resolution of metabolic data, as traditional MFA often provides average fluxes for the entire sample. Advances in imaging techniques are helping to overcome this limitation by enabling spatially resolved metabolic measurements.
