Several factors contribute to uneven illumination in histological studies:
Improper Alignment: Misalignment of the light source, condenser, or objective lens can cause light to scatter unevenly. Lamp Issues: Variations in the intensity of the microscope lamp or an aging light bulb can result in inconsistent light distribution. Condenser Settings: Incorrect condenser settings, such as improper height or aperture diaphragm adjustment, can lead to uneven illumination. Dirt or Debris: Particles on the lenses, light source, or other optical components can obstruct light paths, creating shadows or dark spots.
