Several factors can contribute to inaccurate staining, including:
Improper Fixation: If tissues are not properly fixed, cellular components may degrade, leading to poor staining quality. Incorrect Staining Protocols: Deviations from standard protocols, such as incorrect timing or reagent concentrations, can affect staining results. Reagent Quality: The use of expired or contaminated reagents can result in inconsistent staining. Technical Errors: Human errors, such as incorrect sample preparation or handling, can also lead to staining inaccuracies.
