Halos can be caused by various factors, including:
1. Artifact: Sometimes halos are artifacts resulting from the preparation process of histological slides. These can be due to improper fixation, processing, or staining. 2. Accumulation of Substances: The accumulation of certain substances, such as lipids or mucin, can create a halo effect. 3. Cellular Response: Inflammatory or immune responses can lead to cellular changes that result in halos.
