Probes used in ISH can be classified based on their labeling and composition:
Fluorescent Probes: Probes labeled with fluorescent dyes, enabling detection via fluorescence microscopy. Radioactive Probes: Probes labeled with radioactive isotopes, detected using autoradiography. Enzyme-labeled Probes: Probes tagged with enzymes like alkaline phosphatase or horseradish peroxidase, which produce a colorimetric signal. Oligonucleotide Probes: Short, single-stranded DNA or RNA sequences. RNA Probes: Longer RNA sequences, often referred to as riboprobes.
