There are various types of fluorescence microscopy techniques, each with unique applications:
Widefield Fluorescence Microscopy: Provides a broad illumination of the sample, suitable for general imaging. Confocal Microscopy: Uses a pinhole to eliminate out-of-focus light, providing high-resolution images. Two-Photon Microscopy: Uses two photons for excitation, allowing deeper tissue penetration and reduced phototoxicity. TIRF Microscopy: Excites fluorophores near the sample surface, ideal for studying membrane dynamics.
