Several techniques are employed in histology to study tissues in detail. Common methods include:
Hematoxylin and Eosin (H&E) Staining: This is the most widely used staining technique that highlights tissue architecture and cellular details. Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins within tissue sections, aiding in the identification of disease markers. Electron Microscopy: Provides high-resolution images of tissue ultrastructure, essential for detailed cellular and subcellular analysis. Special Stains: Techniques such as Masson's Trichrome and Periodic Acid-Schiff (PAS) staining are used to highlight specific tissue components like collagen and carbohydrates.
