What are the Techniques to Improve Resolution and Depth of Field?
Various techniques and technologies can be employed to enhance resolution and depth of field in histological studies:
Confocal Microscopy: Utilizes point illumination and spatial pinholes to eliminate out-of-focus light, thus improving resolution and depth of field. Fluorescence Microscopy: Uses fluorescent dyes to label specific structures, enhancing contrast and resolution. Digital image processing and deconvolution: Computational methods to enhance image clarity and depth. Multiphoton Microscopy: Allows deeper tissue penetration with less photodamage, improving depth of field in thicker samples.
