What are the Technical Requirements for SHG Imaging?
SHG imaging requires a specialized microscopy setup. A key component is a femtosecond laser that can produce the high-intensity light needed for SHG. Additionally, the microscope must be equipped with appropriate detectors and filters to capture the emitted second harmonic signal. Advanced imaging systems like multiphoton microscopes are often used for SHG due to their ability to penetrate deeper into tissues.
