The steps involved in vascular perfusion are as follows:
1. Anesthesia: The subject, usually an animal, is anesthetized to minimize pain and stress. 2. Cannulation: A cannula is inserted into a major blood vessel. 3. Initial Flush: The vascular system is flushed with a physiological buffer, such as phosphate-buffered saline (PBS), to clear the blood. 4. Perfusion with Fixative: The fixative solution is then perfused through the vascular system. 5. Post-Perfusion: The tissue is carefully dissected and further processed for histological examination.
