What Are the Steps Involved in Using Phloroglucinol HCl for Staining?
The process of using phloroglucinol HCl for staining involves several steps: 1. Preparation of the Staining Solution: A solution of phloroglucinol in hydrochloric acid is prepared. 2. Application of the Stain: The tissue sample is treated with the phloroglucinol HCl solution. 3. Observation: After application, the sample is observed under a microscope. Lignified tissues will appear red due to the reaction between phloroglucinol HCl and lignin.