The general procedure for sulfide staining involves several key steps:
Fixation: The tissue is fixed to preserve its structure. Deparaffinization: Paraffin-embedded tissue sections are deparaffinized using xylene or alcohol. Hydration: The tissue sections are rehydrated through a series of graded alcohols. Staining: The tissue sections are treated with a sulfide solution to precipitate metal ions. Counterstaining: An optional step to enhance contrast and provide a clearer background. Mounting: The stained tissue sections are mounted on slides for microscopic examination.
