What are the Steps Involved in Sirius Red Staining?
The procedure typically involves the following steps:
Fixation of the tissue sample using formalin or another suitable fixative. Embedding of the sample in paraffin or another embedding medium. Sectioning of the embedded tissue into thin slices. Deparaffinization and rehydration of the tissue sections. Application of Sirius Red dye to the tissue sections. Washing off excess dye. Mounting and examining the stained sections under a microscope.
