1. Tissue Preparation: Samples are typically cryosectioned to preserve enzyme activity. Sections are cut at 5-10 micrometers. 2. Incubation: Sections are incubated in a solution containing a substrate (succinate) and a tetrazolium salt (like NBT). 3. Wash and Mount: After incubation, sections are washed to remove excess substrate and tetrazolium salt and then mounted on slides for microscopic examination.
