Fixation: Tissue samples are usually fixed in formalin to preserve cellular structures. Sectioning: The fixed tissues are embedded in paraffin and sectioned into thin slices using a microtome. Deparaffinization: Sections are treated with xylene to remove paraffin and then rehydrated through a series of alcohol washes. Staining: Sections are incubated with a mixture of potassium ferrocyanide and hydrochloric acid. Counterstaining: Typically, nuclear fast red or safranin is used as a counterstain to provide contrast. Mounting: The stained sections are dehydrated, cleared, and mounted on slides for microscopic examination.
