The process of pathway analysis typically involves several key steps:
Data Collection: Tissue samples are collected and analyzed using techniques such as immunohistochemistry, in situ hybridization, and RNA sequencing. Data Integration: The molecular data obtained is integrated with existing knowledge from databases like KEGG, Reactome, and BioCarta. Pathway Mapping: The integrated data is mapped onto known pathways to identify dysregulated pathways and potential molecular targets. Validation: The findings are validated using additional experimental techniques and bioinformatics tools.
