What Are the Steps Involved in Oil Red O Staining?
The general steps for Oil Red O staining are as follows:
1. Fixation: Tissue samples are fixed, usually in formalin. 2. Cryosectioning: The fixed tissues are frozen and sectioned using a cryostat. 3. Staining: Sections are stained with Oil Red O solution. 4. Rinsing: Excess dye is rinsed off, typically with a water or alcohol solution. 5. Counterstaining: Optionally, sections can be counterstained with hematoxylin to visualize nuclei. 6. Mounting: The stained sections are mounted with an aqueous mounting medium for microscopic examination.
