What Are the Steps Involved in Oil Red O Staining?

The general steps for Oil Red O staining are as follows:

1. Fixation: Tissue samples are fixed, usually in formalin.
2. Cryosectioning: The fixed tissues are frozen and sectioned using a cryostat.
3. Staining: Sections are stained with Oil Red O solution.
4. Rinsing: Excess dye is rinsed off, typically with a water or alcohol solution.
5. Counterstaining: Optionally, sections can be counterstained with hematoxylin to visualize nuclei.
6. Mounting: The stained sections are mounted with an aqueous mounting medium for microscopic examination.