How to Ensure Accurate Results with Oil Red O Staining?
To obtain accurate and reproducible results, consider the following tips:
- Proper Fixation: Ensure the tissue is adequately fixed to preserve the lipid structures. - Fresh Solutions: Use fresh Oil Red O staining solutions to prevent degradation of the dye. - Optimal Staining Time: Standardize staining times to avoid under or over-staining. - Consistent Section Thickness: Use uniform thickness for cryosections to ensure consistent staining.
