The general steps involved in nuclear staining are:
1. Fixation: The tissue sample is fixed using formalin or other fixatives to preserve cellular structures. 2. Embedding: The fixed tissue is embedded in paraffin wax to allow thin sectioning. 3. Sectioning: Thin sections of the tissue are cut using a microtome. 4. Deparaffinization and Rehydration: The sections are deparaffinized and rehydrated to prepare for staining. 5. Staining: The sections are stained with hematoxylin and other dyes. 6. Dehydration and Mounting: The stained sections are dehydrated and mounted for microscopic examination.
