The typical steps for MALDI analysis in histology include: 1. Tissue Preparation: The tissue sample is sectioned and placed on a suitable substrate. 2. Matrix Application: A matrix solution is applied to the tissue section. Common matrices include α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB). 3. Laser Desorption/Ionization: A laser beam is used to irradiate the sample, leading to ionization. 4. Mass Analysis: The generated ions are analyzed using a mass spectrometer, providing information on the mass-to-charge ratio (m/z) of the analytes.
