1. Fixation: Tissue samples are fixed using formalin to preserve morphology. 2. Oxidation: Sections are treated with chromic acid to oxidize tissue components. 3. Rinse: Samples are rinsed with distilled water to remove excess chromic acid. 4. Silver Impregnation: Sections are immersed in a working solution of silver nitrate and methenamine. 5. Rinse and Tone: Excess silver is rinsed off, and gold chloride may be applied to enhance contrast. 6. Reduction: Sodium thiosulfate is used to remove unreacted silver. 7. Counterstaining (optional): Hematoxylin or light green can be used to provide a contrasting background.
