1. Preparation of the Nucleic Acid Probe: Label the nucleic acid with a suitable tag. 2. Protein-Nucleic Acid Binding Reaction: Incubate the labeled probe with the protein extract in a binding buffer. 3. Electrophoresis: Load the reaction mixture onto a non-denaturing gel and run the electrophoresis to separate bound from unbound probes. 4. Detection: Visualize the bands to identify the presence of protein-nucleic acid complexes.