1. Nucleic Acid Probe: This can be a DNA or RNA fragment, labeled with a radioactive, fluorescent, or chemiluminescent tag. 2. Protein Extract: This can be a purified protein or a complex protein mixture extracted from tissue samples. 3. Binding Buffer: Ensures optimal conditions for protein-DNA binding. 4. Non-Denaturing Gel: Polyacrylamide or agarose gel that allows the protein-DNA complex to remain intact during electrophoresis. 5. Detection System: Methods such as autoradiography, fluorescence, or chemiluminescence to visualize the results.
