What are the Steps Involved in Directed Evolution?
The directed evolution process generally involves the following steps:
Generation of Diversity: Introduce genetic variability in the target molecule using methods like mutagenesis or recombination. Selection: Screen and select the variants that exhibit desired traits. Amplification: Amplify the selected variants to create a new pool of candidates for further rounds of evolution. Iteration: Repeat the process to progressively enhance the desired properties of the molecule.
