What are the Steps Involved in Affinity Purification?

1. Preparation of the Affinity Matrix: The ligand, which specifically binds to the molecule of interest, is attached to a solid support, usually beads or a gel matrix.
2. Sample Application: The tissue extract or cell lysate is applied to the matrix.
3. Binding: The target molecule binds to the ligand on the matrix while other components are washed away.
4. Elution: The bound molecule is then eluted from the matrix using a solution that disrupts the ligand-target interaction.
5. Analysis: The purified molecule is collected and can be analyzed further.