What Are the Recent Advances in Fluorescence Multiplexing?

Recent advancements have significantly enhanced the capabilities of fluorescence multiplexing:

Spectral Unmixing: Computational algorithms that separate overlapping fluorescence signals to improve the accuracy of multiplexed imaging.
Super-Resolution Microscopy: Techniques like STED, PALM, and STORM that break the diffraction limit and provide higher resolution images.
Multiplexed Ion Beam Imaging (MIBI): Combining mass spectrometry with fluorescence multiplexing for highly multiplexed and quantitative imaging.
Fluorescent Protein Labels: Using genetically encoded fluorescent proteins to label specific cells or subcellular structures in living tissues.