What Are the Recent Advances in Fluorescence Multiplexing?
Recent advancements have significantly enhanced the capabilities of fluorescence multiplexing:
Spectral Unmixing: Computational algorithms that separate overlapping fluorescence signals to improve the accuracy of multiplexed imaging. Super-Resolution Microscopy: Techniques like STED, PALM, and STORM that break the diffraction limit and provide higher resolution images. Multiplexed Ion Beam Imaging (MIBI): Combining mass spectrometry with fluorescence multiplexing for highly multiplexed and quantitative imaging. Fluorescent Protein Labels: Using genetically encoded fluorescent proteins to label specific cells or subcellular structures in living tissues.
