The process begins with the selection of appropriate fluorochromes that will not interfere with each other's emission spectra. Typically, antibodies conjugated with these fluorochromes are used to target specific antigens within the tissue. After staining, the sample is examined under a fluorescence microscope equipped with filters that can isolate the emission wavelengths of each fluorochrome. Advanced imaging systems can capture these signals separately and then merge them to create a composite image showing the spatial distribution of the different targets.
