Stereology is based on a set of principles that allow for the estimation of three-dimensional structures from two-dimensional sections. Key techniques include:
Point Counting: Estimating volume by counting the number of points that fall within a structure on a grid overlay. Nucleator: Estimating cell size by measuring the distance from a central point to the boundary of the cell. Disector: Estimating the number of objects (e.g., cells) in a volume by comparing sections separated by a known distance.