What Are the Potential Challenges of These Techniques?
Despite their advantages, these new techniques come with certain challenges. For instance, multiplex immunohistochemistry requires careful optimization to prevent cross-reactivity and signal overlap. Multiphoton microscopy demands sophisticated equipment and expertise. Digital pathology raises concerns about data security and standardization. Expansion microscopy involves complex sample preparation. Lastly, single-cell RNA sequencing generates vast amounts of data, necessitating robust computational tools for analysis.