Several techniques are used to verify the monospecificity of an antibody:
Western Blotting: It helps in confirming the antibody binds to a single protein species in a complex mixture. Immunohistochemistry (IHC): This technique is used to visualize the localization of the antibody binding within tissue sections, ensuring that the staining pattern is specific and consistent. Enzyme-Linked Immunosorbent Assay (ELISA): This quantitative method can verify that the antibody binds specifically to its target antigen.
