What Are the Limitations of Using Tritium in Histology?
One limitation of using tritium is its relatively low energy, which can affect the resolution of autoradiographic images. Additionally, the long half-life of tritium (approximately 12.3 years) can lead to prolonged background radioactivity in samples. Another challenge is ensuring accurate quantification of tritium incorporation, as it requires careful calibration and control experiments. Despite these limitations, tritium remains a valuable tool in histological research.
