What are the Limitations of Using Laser Light in Histology?
1. Cost: Equipment such as laser microdissection systems and confocal microscopes can be expensive. 2. Complexity: Operating laser-based systems requires specialized training and expertise. 3. Photobleaching: Prolonged exposure to laser light can lead to photobleaching, where fluorescent dyes lose their ability to emit light. 4. Limited Penetration: Laser light may have limited penetration depth in dense or thick tissues, restricting its use in some applications.
