What Are the Limitations of Using GAPDH in Histology?
Despite its widespread use, there are limitations to using GAPDH as a reference gene:
Expression Variability: Although generally stable, GAPDH expression can vary under certain conditions like hypoxia or during differentiation, which may affect its reliability as a control. Multiple Functions: GAPDH's involvement in various cellular processes means that changes in its expression could be due to factors other than the experimental conditions being studied. Technical Variability: The accuracy of GAPDH detection can be influenced by the quality of antibodies or probes used in techniques like IHC and ISH.
