What are the Limitations of Two Dimensional Representation?
While 2D representations are invaluable, they have certain limitations: 1. Loss of Context: These images provide limited information about the three-dimensional organization of tissues. 2. Artifact Introduction: The preparation process can introduce artifacts that may misrepresent the true structure of the tissue. 3. Limited Depth: Only a thin slice of tissue is examined, potentially missing features present in deeper layers.
