What Are the Limitations of Phase Contrast Microscopy?
Despite its many advantages, phase contrast microscopy has some limitations:
1. Halo Effect: The technique can sometimes produce halos around the edges of specimens, which can obscure details. 2. Limited to Thin Specimens: Thick specimens may not be suitable for phase contrast microscopy as they can cause excessive light scattering. 3. Specialized Equipment: Requires specific phase contrast objectives and condensers, which can be more expensive than standard microscopy equipment.
