What are the Limitations of Multi-Photon Microscopy?
While MPM offers numerous advantages, it also has some limitations:
Cost: The equipment required for MPM is expensive, making it less accessible for some laboratories. Complexity: The technique requires specialized knowledge and training to operate and interpret the results accurately. Resolution: Although MPM provides deep tissue imaging, its resolution is generally lower than that of techniques such as confocal microscopy in superficial layers.
