What are the Limitations of Intracellular Recording?
Despite its advantages, intracellular recording has several limitations. The technique is technically challenging and requires significant skill and precision. The insertion of the microelectrode can damage the cell, potentially altering its physiological properties. Furthermore, the method is generally limited to larger cells due to the delicate nature of the microelectrode. The recorded data can also be influenced by external factors such as temperature and the composition of the extracellular environment.
