Endothelial Layer: This is the innermost layer, consisting of a single layer of endothelial cells that are in direct contact with the blood. These cells form a smooth, frictionless surface. Subendothelial Layer: This middle layer is made up of loose connective tissue containing elastic fibers and collagen. It provides structural support to the endothelial layer. Subendocardial Layer: The outermost layer, which contains Purkinje fibers, nerves, and blood vessels. This layer connects the endocardium to the underlying myocardium.