Fixation: Proper fixation of tissue samples is critical to preserve cellular structures and prevent degradation. Common fixatives include formalin and glutaraldehyde. Embedding: Embedding tissues in paraffin or resin to provide support for sectioning. This step ensures that thin sections can be cut without distortion. Sectioning: Cutting thin sections (typically 3-5 micrometers thick) using a microtome. This allows for the detailed examination of tissue architecture. Staining: Application of various stains (e.g., Hematoxylin and Eosin) to highlight different tissue components. Standardized staining protocols ensure uniform results. Quality Control: Implementation of quality control measures, such as regular calibration of equipment and validation of reagents, to maintain high standards.
