- Chromosome Alignment: Chromosomes align along the metaphase plate, ensuring that each daughter cell will receive one copy of each chromosome. - Spindle Apparatus: The spindle fibers attach to the centromeres of the chromosomes, playing a crucial role in pulling them apart during the next stage, anaphase. - Centrosomes: These organelles, located at opposite poles of the cell, organize the spindle fibers and help in the alignment process.