What Are the Key Elements of Quality Control in Histology?
Several elements are critical to effective QC in histology:
Sample Collection: Proper collection and handling of tissue samples are fundamental. Ensuring samples are not contaminated and are preserved correctly is crucial. Fixation: Adequate fixation is necessary to preserve tissue morphology and prevent autolysis. Formalin is commonly used, and the fixation time should be optimized. Embedding: Proper embedding of tissue samples in paraffin wax ensures that they are properly oriented and supported for sectioning. Sectioning: Uniform and thin sections are critical for accurate staining and microscopic evaluation. Staining: Standardized staining protocols, such as Hematoxylin and Eosin (H&E), need to be followed to achieve consistent results. Microscopic Examination: Regular calibration of microscopes and training of personnel ensure accurate interpretation of slides.
