In histological sections, lipofuscin appears as yellow-brown, autofluorescent granules within the cytoplasm. It can be visualized using various staining techniques such as Hematoxylin and Eosin (H&E) staining, Periodic Acid-Schiff (PAS) staining, and Sudan Black staining. Lipofuscin's autofluorescence is another key identifying feature, which is particularly useful in fluorescence microscopy.
