Poor buffer control can lead to several issues, including:
- Inconsistent Staining: Variations in pH can cause irregular staining patterns, making it difficult to interpret tissue structures. - Loss of Antigenicity: In immunohistochemistry, improper pH levels can result in the loss of antigen-antibody binding, leading to false-negative results. - Tissue Damage: Extreme pH levels can cause damage to tissue samples, compromising their integrity and hindering accurate analysis.
