What Are the Common Techniques Used in Histological Therapeutic Evaluation?
Several methods are employed to assess therapeutic outcomes in histology:
Hematoxylin and Eosin (H&E) Staining: This standard technique provides an overview of tissue morphology and cellular details. Immunohistochemistry (IHC): IHC uses antibodies to detect specific proteins, helping to identify biomarkers associated with disease or therapeutic response. In Situ Hybridization (ISH): ISH allows for the detection of specific nucleic acids within tissues, useful for identifying genetic changes or pathogen presence. Electron Microscopy (EM): EM provides ultrastructural details, essential for studying cellular organelles and fine tissue architecture.
