What are the Common Staining Protocols Using Light Green?
One of the most well-known protocols involving Light Green is the Masson's Trichrome stain. This staining technique is used to differentiate between muscle, collagen, and fibrin in tissue sections. In Masson's Trichrome, Light Green serves as a counterstain to highlight the connective tissue in green, contrasting with the red and blue hues of other cellular components.
Another common protocol is the Papanicolaou stain (Pap stain), widely used in cytology for examining cells from bodily fluids. Light Green is part of the Pap stain's multichromatic regimen, helping to stain the cytoplasm of cells, thereby facilitating the identification of cellular abnormalities.
