Several factors contribute to diagnostic inaccuracies in histology:
1. Sample Collection and Handling: Poorly collected or mishandled samples can lead to distortions, contamination, or degradation, affecting the accuracy of the analysis.
2. Tissue Processing: Errors during tissue processing, such as improper fixation, embedding, or sectioning, can result in artifacts that obscure critical details.
3. Staining Techniques: Inconsistent or incorrect staining can make it difficult to distinguish between different cell types and structures.
4. Observer Error: Human error is a significant factor, including misinterpretation of histological features or overlooking subtle abnormalities.
5. Technical Limitations: Limitations of the microscopic or imaging equipment can also affect the quality of the diagnosis.
