Several methods are employed for cell isolation, including:
1. Enzymatic Digestion: Uses enzymes like trypsin or collagenase to break down the extracellular matrix, releasing individual cells. 2. Mechanical Dissociation: Involves physically disrupting tissues using tools like scalpels or homogenizers. 3. Density Gradient Centrifugation: Separates cells based on their density. 4. FACS: Utilizes fluorescent markers and lasers to sort cells. 5. Magnetic-Activated Cell Sorting (MACS): Uses magnetic particles attached to antibodies to separate specific cell types.
