What are the Common Imaging Techniques Used in Histology?
Several imaging techniques are employed in histology to obtain detailed views of tissue samples:
Light Microscopy: The most fundamental and widely used method, relying on visible light to magnify tissue samples. Fluorescence Microscopy: Utilizes fluorescent dyes that bind to specific cellular components, providing high-contrast images of structures and functions. Electron Microscopy: Offers extremely high resolution by using electron beams, allowing for the visualization of ultrastructural details. Confocal Microscopy: Employs laser light to create sharp, three-dimensional images of tissues, minimizing out-of-focus light. Multiphoton Microscopy: A variant of fluorescence microscopy that uses longer wavelength light, enabling deeper tissue penetration with reduced phototoxicity.
