Ultramicrotomy presents several challenges, including:
Specimen Preparation: Proper fixation and embedding are critical for producing high-quality sections. Inadequate preparation can lead to artifacts and poor section quality. Knife Quality: The cutting edge of the knife must be extremely sharp and free from damage. Even minor imperfections can result in tearing or compression of the sections. Section Thickness: Maintaining consistent section thickness is challenging but essential for accurate analysis. Variations in thickness can affect the interpretation of the data.
