Despite its advantages, 3D microscopy also presents several challenges, including:
Technical Complexity: The operation and interpretation of 3D imaging systems require specialized knowledge and training. High Cost: Advanced 3D microscopy equipment can be expensive, limiting accessibility for some laboratories. Data Management: 3D datasets are significantly larger than 2D images, necessitating robust data storage and processing capabilities. Sample Preparation: Preparing samples for 3D imaging can be more complex and time-consuming than for 2D microscopy.
